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Arial, single-spaced, justified, 1-inch margins, page number (bottom, right). Use scientific terminology and a professional tone with proper spelling, grammar, punctuation, syntax).
Title: this title might be the same or similar to a title used in the Hypothesis and Background Statement assignment. That is to be expected!
1 specific aim that states clearly an experimental approach to addressing your research hypothesis. Your hypothesis should be focused on the gap in knowledge for the research topic, thus this specific aim should describe a way of addressing the gap in knowledge.
The aim should briefly describe how you will test each your hypothesis using experiments (e.g. RNA collected from patients will be analyzed by qRT-PCR for a specific gene mutation or glucose tolerance tests will be completed prior to and after the high intensity interval training program). Describe what will happen if your hypothesis is supported (i.e. expected outcomes). You may include a cartoon, schematic or illustration to assist with describing your specific research aim. You should use citations in this section where appropriate.
Methods Section
This section should be 1-2 paragraphs in length. Focus on describing the model you will use if you plan to use one and why this model is important. Describe the necessary ethical considerations (approvals from IRB or IACUC). If you are working with patients, who will you exclude/include and why? How will you recruit participants? Describe simply the equipment and/or resources that you will need to conduct the research. For each major experiment, describe what you will be measuring and how you will collect the data. Explain what controls you will incorporate for specific variables. If it assists in describing your methods, you may use citations in this section.
References
Using JAMA format, use in-text citations throughout the writing where appropriate. When you are describing a method, you should cite literature where this method has been used previously. You must have a minimum of 5 citations, including a mixture of both primary and secondary sources. Remember that it is always best to cite the original source of data or a new concept (i.e. it is better to cite the original primary source, which discovered the data than just a review describing the discovery).

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